1886-1888 German scientist Herriger (H. Hellriegal), under the condition of sand culture, proved that leguminous plants only form Rhizobia

　　Nitrogen in a fixed atmosphere. 1888 Dutch scholar Bayesian (M. W. Beijerinck) separates Rhizobia, a breakthrough in microbial fertilizers. It is now clear that it is the role of Rhizobia. The discovery of these bacteria prompted the first American company, Turner, to produce and sell soil-bacterial inoculation in 1898. Since then, a number of bacterial formulations have been used for the seed and coating of soil and crop seeds.

　　In the the 1920s, new microbial formulations were used for soil and crops in the field, but the results were not satisfactory. In the 1940s, USDA issued a permit for biological pesticides, and more than 20 different microbial products have been used for this purpose.

　　In the 1937, the Soviet microbiological biologist Krasinikov and the Secret Sioux Gold developed "nitrogen-fixing agent". Thus creating a precedent for bacterial fertilizers, for various reasons, the microbial fertilizer has stopped the mass production. Around 1940, Asia developed a bio-fertilizer based on blue bacteria (algae) for paddy fields. Now it still plays a huge role in sustainable agriculture.

　　Microbial preparations continue to evolve regardless of the history of biological fertilizers. Since the the 1980s, there has been a great deal of attention to bio-fertilizers for the environment and crops, because such products can effectively solve some problems, especially pollution-free and environment-friendly. Therefore, it is very difficult to develop a kind of capability which has both the function of fertilizer and the ability to eliminate environmental pollution.